Sıçan Ovaryumunda Bisfenol-A Maruziyetinin Neden Olduğu Oksidatif Stres ve Olası Histopatolojik Değişikliklere Karşı Quercetin'in Etkisi
Effect of quercetin against BPA exposure in the ovary
DOI:
https://doi.org/10.5281/zenodo.10019742Anahtar Kelimeler:
Ovaryum- Oksidatif stres- Bisfenol A- Kuersetin- SıçanÖzet
ÖZ
Amaç: Endokrin bozucu kimyasallar üreme sistemi üzerinde olumsuz etkilere sahip olabilir. Bu kimyasallardan biri olan bisfenol A (BPA), östrojen benzeri etkileri nedeniyle, oksidatif stresi tetiklediğine ve yumurtalık fonksiyonlarını potansiyel olarak bozarak özellikle kadınlarda doğurganlığın azalmasına yol açtığına inanılmaktadır.
Gereç ve Yöntem: Bu çalışmada, 32 dişi Wistar albino sıçan kullanıldı, ve kontrol (mısır yağı, p.o.), BPA (10 mg/kg p.o.), Q (15 mg/kg i.p.) ve BPA+Q olmak üzere 4 grup oluşturuldu. Sıçanlar sakrifiye edildikten sonra ovaryum dokularında histopatolojik değişimler ve Malondialdehyde (MDA), Catalase (CAT), advanced oxidation protein products (AOPP), ve glutathione (GSH) düzeyleri spektrofotometrik olarak analiz edildi.
Bulgular: Toplam 14 gün süren çalışmanın sonuçları, BPA'nın dişi sıçanların yumurtalık dokularında değişikliklere neden olduğunu göstermiştir. Biyokimyasal ve histopatolojik analiz sonuçları, kuersetin uygulamasının BPA'nın olumsuz etkisini iyileştirdiğini belirledi. BPA uygulaması, oksidatif stres biyobelirteçleri olan MDA ve AOPP seviyelerinde önemli bir artış göstermiştir. Ayrıca, antioksidan enzim seviyeleri (CAT ve GSH quercetin uygulaması ile artmıştır.
Sonuç: BPA dişi sıçanların yumurtalık dokularında oksidatif stresi indükleyerek histopatolojik değişikliklere yol açabilir. Öte yandan, kuersetin tedavisi, BPA'nın üreme sistemi üzerindeki zayıflatıcı etkilerini önemli ölçüde azaltarak koruyucu bir etki göstermiştir.
Anahtar Kelimeler: Ovaryum, Oksidatif stres, Bisfenol-A, Quercetin, Sıçan.
İndirmeler
Referanslar
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