Sensitivity and Specificity of M2-3E (BPO), Anti-GP210, and Anti-SP100 Antibodies in M2-Positive Patients and Their Importance in the Diagnosis of PBC
SEROLOGIC TESTS IN PRIMARY BILIARY CIRRHOSIS
DOI:
https://doi.org/10.5281/zenodo.17456016Keywords:
Primary Biliary Cirrhosis, Anti Mitochondrial Antibody, Anti gp210Abstract
PURPOSE: The aim of this study was to support the diagnosis of primary biliary cirrhosis (PBC) by determining the sensitivity and the specifıcity of anti- gp210 and anti-Sp100 antibodies in patients diagnosed with PBC and had positive AMA-M2. MATERIAL AND METHODS: Total of 54 patients, 40 females and 14 males, who were AMA-M2 positive and presented to the Ankara Numune Research and Training Hospital Microbiology Laboratory were included in the study. The patients were grouped as those diagnosed with PBC and those not. The anti-gp210 and anti-Sp100 antibodies were studied in microbiology and clinical microbiology laboratories with EUROIMMUN (Germany) test kits in accordance with the extractable nuclear antigen (ENA) profile 1 Euroline study protocols as defined by the manufacturer. RESULTS: We found that the AMA, gp210 and Sp100 results were not statistically signifıcant in differentiating patients with and without primary biliary cirrhosis (p=0.633, p=1.000 and p=0.276 respectively). On the other hand M2-3E (BPO) results were statistically significant in differentiating patients with and without primary biliary cirrhosis (p=0.033) CONCLUSION: We concluded that anti-gp210 and anti-Sp100 antibodies cannot be used as diagnostic markers far PBS. The sensitivity of AMA and M2-3E (BPO) markes far PBS was high.Downloads
References
1. Kaplan MM, Gershwin ME. Primary biliary cirrhosis. N Engl J Med. 2005;353(12):1261-73.
2. Shah SK, Bowlus CL. Autoimmune Markers in Primary Biliary Cholangitis. Clin Liver Dis. 2024;28(1):93-101.
3. Smyk D, Cholongitas E, Kriese S, Rigopoulou EI, Bogdanos DP. Primary biliary cirrhosis: family stories. Autoimmune diseases
2011;2011(1):189585.
4. Pandit S, Samant H. Primary Biliary Cholangitis. In: StatPearls. Treasure Island (FL): StatPearls Publishing; 2017.
5. Younossi ZM, Bernstein D, Shiffman ML, et al. Diagnosis and Management of Primary Biliary Cholangitis. Am J Gastroenterol.
2019;114(1):48-63.
6. Kim KA, Jeong SH. The diagnosis and treatment of primary biliary cirrhosis. Korean J Hepatol. 2011;17(3):173-9.
7. Zhang Y, Jin Y, Liu Y, Wang M, Xu A. Diagnostic value of quantitative chemiluminescence immunoassay for anti-gp210 and anti-sp100 antibodies in primary biliary cholangitis. Clin Chem Lab Med. 2024;62(8):193-6.
8. Ben Lamine Z, Ben Jazia I, Ben Ahmed M, et al. Anti-gp210 and anti-Sp100 antibodies in primary biliary cholangitis. Arab J Gastroenterol. 2021;22(4):316-20.
9. Jaskowski TD, Nandakumar V, Novis CL, Palmer M, Tebo AE. Presence of anti-gp210 or anti-sp100 antibodies in AMA positive
patients may help support a diagnosis of primary biliary cholangitis. Clin Chim Acta. 2023;540:117219.
10. de Liso F, Matinato C, Ronchi M, Maiavacca R. The diagnostic accuracy of biomarkers for diagnosis of primary biliary cholangitis
(PBC) in anti-mitochondrial antibody (AMA)-negative PBC patients: a review of literature. Clin Chem Lab Med. 2017;56(1):25-31.
11. Hirschfield GM, Dyson JK, Alexander GJM, et al. The British Society of Gastroenterology/UK-PBC primary biliary cholangitis treatment
and management guidelines. Gut. 2018;67(9):1568-94.
12. Xu J, Zhang H, Wang C, et al. Increased sensitivity of gp210 autoantibody detection using a newly designed gp210 antigen. J Immunol Methods. 2022;501:113211.
13. Mytilinaiou MG, Meyer W, Scheper T, et al. Diagnostic and clinical utility of antibodies against the nuclear body promyelocytic
leukaemia and Sp100 antigens in patients with primary biliary cirrhosis. Clin Chim Acta. 2012;413(15-16):1211-6.
14. Wang C, Qin Z, Zhang M, et al. Autoantibodes to GP210 are a metric for UDCA responses in primary biliary cholangitis. J Transl
Autoimmun. 2024;8:100239.
15. Muratori P, Muratori L, Ferrari R, et al. Characterization and clinical impact of antinuclear antibodies in primary biliary cirrhosis. Am J
Gastroenterol. 2003;98(2):431-7.
16. Parés A. Primary biliary cholangitis. Colangitis biliar primaria. Med Clin (Barc). 2018;151(6):242-9.
17. Rigopoulou EI, Bogdanos DP. Role of autoantibodies in the clinical management of primary biliary cholangitis. World J Gastroenterol. 2023;29(12):1795-810.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 FİGEN TÜREN, ALTAN AKSOY
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
